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KMID : 0380020100250020199
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 2 p.199 ~ p.204
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Production of Repetitive Polypeptides for an Efficient DNA Analysis on a Microchip
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Yi Hyeon-Jin
Choi Seok-Jin
Seo Tae-Seok
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Abstract
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We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.
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KEYWORD
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repetitive polypeptide, end-labeled free-solution electrophoresis (ELFSE), drag-tag, capillary electrophoresis, microchip
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